Generator

Part:BBa_K223062:Design

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


TolC and Hemolysin B, C and D gene complex


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1572
    Illegal suffix found in sequence at 1523
    Illegal EcoRI site found at 1594
    Illegal EcoRI site found at 4669
    Illegal XbaI site found at 4684
    Illegal SpeI site found at 2354
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 4621
    Illegal SpeI site found at 8342
    Illegal PstI site found at 362
    Illegal PstI site found at 4635
    Illegal PstI site found at 5855
    Illegal PstI site found at 8249
    Illegal PstI site found at 8356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 1594
    Illegal EcoRI site found at 4669
    Illegal SpeI site found at 1524
    Illegal SpeI site found at 2354
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 4621
    Illegal SpeI site found at 8342
    Illegal PstI site found at 362
    Illegal PstI site found at 1538
    Illegal PstI site found at 4635
    Illegal PstI site found at 5855
    Illegal PstI site found at 8249
    Illegal PstI site found at 8356
    Illegal NotI site found at 27
    Illegal NotI site found at 1531
    Illegal NotI site found at 1578
    Illegal NotI site found at 4628
    Illegal NotI site found at 4675
    Illegal NotI site found at 8349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 1594
    Illegal EcoRI site found at 4669
    Illegal BamHI site found at 4458
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 1524
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 1594
    Illegal EcoRI site found at 4669
    Illegal XbaI site found at 1587
    Illegal XbaI site found at 4684
    Illegal SpeI site found at 2354
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 4621
    Illegal SpeI site found at 8342
    Illegal PstI site found at 362
    Illegal PstI site found at 4635
    Illegal PstI site found at 5855
    Illegal PstI site found at 8249
    Illegal PstI site found at 8356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 1594
    Illegal EcoRI site found at 4669
    Illegal XbaI site found at 36
    Illegal XbaI site found at 1587
    Illegal XbaI site found at 4684
    Illegal SpeI site found at 1524
    Illegal SpeI site found at 2354
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 4621
    Illegal SpeI site found at 8342
    Illegal PstI site found at 362
    Illegal PstI site found at 1538
    Illegal PstI site found at 4635
    Illegal PstI site found at 5855
    Illegal PstI site found at 8249
    Illegal PstI site found at 8356
    Illegal AgeI site found at 662
    Illegal AgeI site found at 1369
    Illegal AgeI site found at 6027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1776
    Illegal BsaI site found at 2477
    Illegal SapI.rc site found at 7867


Design Notes

n/a


Source

Synthesized by Stanford iGEM 2009

References